Differential effects of follistatin on porcine oocyte competence and cumulus cell gene expression in vitro          下载免费PDF全文

Z Guo  D Liu  G Liu  L Lv  Y Yang  B Fu  L Wang  Z Liu  H He  H Wu 《Reproduction in domestic animals》2018,53(1):3-10

  相似文献   

Comparative expression profile of microRNAs and piRNAs in three ruminant species testes using next‐generation sequencing          下载免费PDF全文

H Wang  J Zhong  Z Chai  J Zhu  J Xin 《Reproduction in domestic animals》2018,53(4):963-970

microRNA (miRNA) and piwi‐interacting RNA (piRNA) are two classes small non‐coding regulatory RNAs that play crucial roles in multiple biological processes such as spermatogenesis. However, there are no published studies on conjoint analysis of miRNA and piRNA profiles among cattle, yak and their interspecies (the dzo) using sequencing technology. Next‐generation sequencing technology was used to profile miRNAs and piRNAs among those three ruminants to elucidate their functions. A total of 119, 14 and six differentially expressed miRNAs were obtained in cattle vs. dzo, cattle vs. yak and yak vs. dzo comparison groups, while there were 873, 1,065 and 1,158 differentially expressed piRNAs in those three comparison groups. The expression of three miRNAs was validated in the three ruminants, and the results suggested that the miRNA expression profiles data could represent actual miRNA expression levels. Moreover, the putative targets of differentially expressed miRNAs were predicted by their own genome, it is worth to note that both the cattle and yak genome were used for dzo, then the targets were subjected to GO enrichment and KEGG pathway analysis, revealing the likely roles for these differentially expressed miRNAs in spermatogenesis. In conclusion, this study provided a useful resource for further elucidation of the miRNAs and piRNAs regulatory roles in spermatogenesis. It may also facilitate the development of therapeutic strategies for dzo reproduction research.  相似文献   

Comparative analysis of ovarian transcriptomes between prolific and non‐prolific goat breeds via high‐throughput sequencing          下载免费PDF全文

X‐D Zi  J‐Y Lu  H Zhou  L Ma  W Xia  X‐R Xiong  D‐L Lan  X‐H Wu 《Reproduction in domestic animals》2018,53(2):344-351

  相似文献   

Disaccharide combinations and the expression of enolase3 and plasma membrane Ca2+ ATPase isoform in sturgeon sperm cryopreservation          下载免费PDF全文

P Li  H Du  XM Qiao  ZG Liu  QW Wei 《Reproduction in domestic animals》2018,53(2):472-483

Acipenser sinensis and Acipenser dabryanus are critically endangered species, so germplasm conservation via cryopreservation of sperm is necessary. Disaccharides can act as membrane‐impermeable cryoprotectants, and enolase3 (ENO3) and plasma membrane Ca²⁺ ATPase isoform (PMCA2) are proteins associated with sperm quality. We considered seven characteristics of sperm quality in cultured brood stock from A. sinensis and A. dabryanus. We tested use of sucrose or trehalose alone and in combination at different concentrations for cryopreservation of A. dabryanus sperm. A low concentration of sucrose plus trehalose (S₁₅T₁₅) was optimal. Mixing of the extender with sucrose, lactose, or trehalose alone or with pairwise mixtures revealed that a mixture of lactose and trehalose (L₁₅T₁₅) gave the best results for both A. sinensis and A. dabryanus. Enolase3 and PMCA2 expression levels were measured in cryopreserved A. sinensis sperm via Western blotting. Relative ENO3 and PMCA2 expression levels were examined, and the relationship between disaccharide composition, sperm quality and protein expression was explored in A. sinensis. The results showed that relative ENO3 and PMCA2 expression levels were the highest at L₁₅T₁₅ in cryopreserved A. sinensis sperm. There were significant positive correlations between ENO3 expression and percentage membrane integrity, and between PMCA2 expression and sperm motility parameters (percentage of motile sperm, curvilinear velocity, straight‐line velocity and average path velocity; p < .05) in cryopreserved A. sinensis sperm. Our results indicate the optimal disaccharide combination and concentrations for cryopreservation of A. sinensis and A. dabryanus sperm and suggest that ENO3 and PMCA2 expression levels could serve as a valuable indicator of sperm quality in A. sinensis.  相似文献   

Organic zinc and copper supplementation on antioxidant protective mechanism and their correlation with sperm functional characteristics in goats          下载免费PDF全文

M Narasimhaiah  A Arunachalam  S Sellappan  VK Mayasula  PR Guvvala  SK Ghosh  V Chandra  J Ghosh  H Kumar 《Reproduction in domestic animals》2018,53(3):644-654

Trace minerals feeding had significant effects on sperm production and fertility with better absorption and proper utilization within the body for optimum reproductive function. Several studies have shown that more influenced trace elements in the diets of animals are copper (Cu) and zinc (Zn). Bucks showing deficiency of this mineral might affect the quality of semen production which in turn would affect the fertility. This experiment was thus designed to test the effects of organic Cu and Zn supplementation on antioxidants enzyme activities and sperm functional attributes in fresh semen of bucks. Forty bucks (n = 40, Aged 5 months) were assigned to ten groups of four animals in each group, supplemented (for a period of 8 months) with different levels of organic Zn: 20 mg (T2), 40 mg (T3) and 60 mg (T4), organic Cu: 12.5 mg (T5), 25 mg (T6), 37.5 mg (T7) and combined organic Zn and Cu: 20 + 12.5 mg (T8), 40 + 25 mg (T9), 60 + 37.5 mg (T10), respectively, per kg dry matter and no additional mineral diet (control; T1). One hundred and sixty semen samples were collected through electro‐ejaculator and analysed for sperm quantity, quality, acrosome intactness and plasma membrane integrity and correlated with the catalase, superoxide dismutase, glutathione peroxidase and glutathione reductase enzyme activities in seminal plasma. The results indicated organic Cu and zinc supplemented bucks produced more sperm cells, had higher sperm concentrations, maintained higher (p < .01) sperm livability, plasma membrane and acrosome integrities, more motility and velocity. The increased antioxidant enzyme activities, reduced oxidative stress and lowered lipid peroxidation were positively correlated (p < .05) with the sperm functional attributes. In conclusion, organic Cu and Zn supplement to male goats showed protective roles against oxidative damage and maintained better fresh semen characteristics.  相似文献   

An unresponsive progressive pustular and crusting dermatitis with acantholysis in nine cats          下载免费PDF全文

Catherine A. Outerbridge  Verena K. Affolter  Leslie A. Lyons  Samantha L. Crothers  Andrea T. H. Lam  Terri E. Bonenberger  Stephen D. White 《Veterinary dermatology》2018,29(1):81-e33

  相似文献   

Caecal intussusceptions and typhlocolitis in horses with severe Gastrodiscus aegyptiacus infestation          下载免费PDF全文

Z. Gratwick  C. Donnellan  P. C. Page  A. Viljoen  J. Williams  C. H. Lyle 《Equine Veterinary Education》2018,30(5):247-254

The intestinal trematode Gastrodiscus aegyptiacus (G. aegyptiacus) has been recognised in equids around the world for many years, but its pathogenicity is yet to be confirmed. This report describes seven cases of severe G. aegyptiacus infestation, including six cases of caecal intussusception.  相似文献   

Acupuncture and traditional Chinese veterinary medicine for treatment of a mare with rectal paralysis          下载免费PDF全文

M. M. Sleeper  K. Fishman  R. J. MacKay  M. Brown  A. G. Bauck  H. Xie 《Equine Veterinary Education》2018,30(9):460-463

An 8‐year‐old Appaloosa mare with rectal paralysis due to a cosmetic ethanol ‘tail block’ was treated with traditional Chinese veterinary medicine treatments including acupuncture and herbal medicine. Her rectal and tail tone gradually improved after the treatment. At 4 months after initial presentation, the mare was able to produce faecal piles on a regular basis, and manual evacuations were no longer needed. Significant improvement was within 30 days of beginning treatment. At 8 months, the owner indicated that the mare had normal defaecation, was able to swish the tail from side to side and lift the tail to urinate, and had no evidence of straining to defaecate or colic.  相似文献   

Influence of the root endophyte Piriformospora indica on the plant water relations,gas exchange and growth of Chenopodium quinoa at limited water availability          下载免费PDF全文

S. Hussin  W. Khalifa  N. Geissler  H.‐W. Koyro 《Journal of Agronomy and Crop Science》2017,203(5):373-384

This study aimed to evaluate the ability of Piriformospora indica to colonize the root of Chenopodium quinoa and to verify whether this endosymbiont can improve the growth, performance and drought resistance of this species. The study delivered, for the first time, evidence for successful colonization of P. indica in quinoa. Hence, pot experiment was conducted in the greenhouse, where inoculated and non‐inoculated plants were subjected to ample (40%–50% WHC) and deficit (15%–20%WHC) irrigation treatments. Drought adversely influenced the plant growth, leading to decline the total plant biomass by 74%. This was linked to an impaired photosynthetic activity (caused by lower g_s and C_i/C_a ratio; stomatal limitation of photosynthesis) and a higher risk of ROS production (enhanced ETR/A_gross ratio). P. indica colonization improved quinoa plant growth, with total biomass increased by 8% (controls) and 76% (drought‐stressed plants), confirming the growth‐promoting activity of P. indica. Fungal colonization seems to diminish drought‐induced growth hindrance, likely, through an improved water balance, reflected by the higher leaf ψ_w and g_s. Additionally, stomatal limitation of photosynthesis was alleviated (indicated by enhanced C_i/C_a ratio and A_net), so that the threat of oxidative stress was minimized (decreased ETR/A_gross). These results infer that symbiosis with P. indica could negate some of the detrimental effects of drought on quinoa growth, a highly desired feature, in particular at low water availability.  相似文献   

Estimated Breeding Value in Iranian Buffaloes     

M. R. Sanjabi  F. Alemi  H. R. Naderfard 《农业科学与技术》2011,(4):570-574

This study was conducted from 1992-2002 on 65,534 individual milk records and fat percentage in 473 herds, in six provinces of Khuzestan, Mazndaran, W. Azerbaijan, E. Azerbaijan, Ardabil and Gilan. The data was analyzed by SAS sotfware using GLM procedure. The heritability and breeding value of 1,195 animals were calculated by DFRML procedure. The average milk yield per lactation, days of lactation, fat percentage and LSM of fat percentage were 1,513 kg, 202 days, 5.04 and 6.77, respectively. The estimated heritability of milk was 0.16. The LSM of average milk production in the provinces of Gilan, Mazandaran, E. Azerbaijan, W. Azerbaijan, Khuzestan and Ardabil were： 1,452, 1,586, 1,382, 1,183, 2,135 and 1,189 kg, respectively. These results indicated that Khuzestan province has the highest potential in the field of milk production. The top five highest breeding value bulls have been introduced to artificial insemination＇s station in the city of Uremia.  相似文献